mouse colon carcinoma cell line mc38  (Absolute Biotech Inc)

Journal: bioRxiv

Article Title: Microdissected tumor cuboids: a microscale cancer model that retains a complex tumor microenvironment

doi: 10.1101/2024.03.22.586189

Figure Lengend Snippet: Cytokine/chemokine secretion in cuboids grown in 96 well plates, measured by Luminex. (A) Baseline secretion from Py8119 mouse breast cancer cuboids (15 of 31 cytokines, 2-6 cuboids/well). (B) Baseline secretion from MC38 mouse colon cancer cuboids (29 cytokines, 4-8 cuboids/well). (C) Cytokine response of Py8119 cuboids to LPS stimulation (2-6 cuboids/well). (D) Baseline secretion from human colorectal cancer metastasis cuboids (4-6 cuboids per well, day 5). Individual replicates shown.

Article Snippet: The MC38 syngeneic mouse colon carcinoma cell line (Kerafast, ENH204-FP) was cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

Techniques: Luminex

Journal: Cell reports

Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation

doi: 10.1016/j.celrep.2021.109587

Figure Lengend Snippet: (A) Representative confocal images of bone-marrow-derived macrophages (BMDMs, magenta) incubated with MC38-hHER2 cells (green), either with or without tumor targeting anti-hHER2 antibody opsonization. White arrows within insets indicate phagocytosed MC38-hHER2 cells. Scale bar is 50 μm. (B) Quantification of MC38-hHER2 tumor cell phagocytosis by BMDMs. MC38-hHER2 tumor cells were opsonized with a range of anti-hHER2 antibody concentrations (0–5 μg/mL). Each condition is the average of three independent experiments representing >150 total cells. Bars represent means ± SEM. Conditions were compared with two-tailed Student’s t test. **p < 0.01, ***p < 0.001. (C) Fluorescent confocal images of fixed BMDMs engaging with MC38-hHER2 cells (green) opsonized with different concentrations of anti-hHER2 antibody (0,0.5, and 5 μg/mL, magenta). Phosphorylation visualized via anti-phosphotyrosine (red). BMDM outlines are shown in white dotted lines. Areas of high anti-hHER2 enrichment are highlighted with white arrows. Scale bar is 5 μm. (D) Anti-SIRPα blocking antibody 18a interrupting SIRPα binding to CD47 (left panel). MC38-hHER2 cells incubated with a range of anti-hHER2 antibody concentrations (0–5 μg/mL) were added to BMDMs in the presence or absence of 5 μg/mL of 18a antibody. Average tumor cell phagocytosis was quantified (right panel). Each condition is the average of three independent experiments representing >150 total cells. Bars represent means ± SEM. Conditions were compared with two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: MC38 mouse colon carcinoma cell line , Aduro Biotech , N/A.

Techniques: Derivative Assay, Incubation, Two Tailed Test, Phospho-proteomics, Blocking Assay, Binding Assay

Journal: Cell reports

Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation

doi: 10.1016/j.celrep.2021.109587

Figure Lengend Snippet:

Article Snippet: MC38 mouse colon carcinoma cell line , Aduro Biotech , N/A.

Techniques: Mutagenesis, Blocking Assay, Virus, Recombinant, Cell Culture, Derivative Assay, Plasmid Preparation, Software